Adenovirus Transduction and Endogenous Protein Knockdown by siRNA Transfection in HeLa-RFP-H2B Cells Using CaPi Precipitates
7:46
Live Cell Imaging of Mitotic Cells and Data Analysis
9:36
Results: Adenofection Effectively Introduces Proteins of Interest without Affecting Mitosis
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Conclusion
필기록
The overall goal of this method is to enable depletion rescue experiments to be performed under conditions that preserve cellular integrity and protein homeostasis. For functional analyses of biological processes at the single cell level. This met
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We describe a method for depletion-rescue experiments that preserves cellular integrity and protein homeostasis. Adenofection enables functional analyses of proteins within biological processes that rely on finely tuned actin-based dynamics, such as mitotic cell division and myogenesis, at the single-cell level.