JoVE Logo
Faculty Resource Center

Sign In

A Submerged Filter Paper Sandwich for Long-term Ex Ovo Time-lapse Imaging of Early Chick Embryos

DOI :

10.3791/54636-v

7:29 min

December 28th, 2016

December 28th, 2016

21,782 Views

1Department of Orthopaedic Surgery, VU University Medical Center Amsterdam, MOVE Research Institute Amsterdam, 2Department of Anatomy, Embryology & Physiology, Academic Medical Center Amsterdam

Here, we present a new technique to culture chick embryos ex ovo for time-lapse imaging using transmitted light and fluorescence microscopy. The submerged filter paper sandwich is a variant of the well-established filter paper carrier technique1 that allows for the culturing of chick embryos fully-submerged in a liquid culture medium.

Tags

Submerged Filter Paper Sandwich

-- Views

Related Videos

article

Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach

article

Optimized Ex-ovo Culturing of Chick Embryos to Advanced Stages of Development

article

Analyzing In Vivo Cell Migration using Cell Transplantations and Time-lapse Imaging in Zebrafish Embryos

article

Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning

article

An Ex Vivo Method for Time-Lapse Imaging of Cultured Rat Mesenteric Microvascular Networks

article

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

article

A Simple Chamber for Long-term Confocal Imaging of Root and Hypocotyl Development

article

Long-term Live Imaging of Drosophila Eye Disc

article

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

article

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved