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Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

DOI :

10.3791/56214-v

August 9th, 2017

August 9th, 2017

7,316 Views

1Department of Ophthalmology and Visual Sciences, Kellogg Eye Center, University of Michigan

A combination of the advanced optical techniques of laser scanning microscopy with long wavelength multi-photon fluorescence excitation was implemented to capture high-resolution, three-dimensional, real-time imaging of neural crest migration in Tg(sox10:EGFP) and Tg(foxd3:GFP) zebrafish embryos.

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Multi photon Time Lapse Imaging

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