Cell Surface Staining for Fluorescence-Activated Cell Sorting (FACS)
4:09
Plasmacytoid Cell FACS Purification
5:48
Results: IFNalpha Production by FACS-Sorted pDC
7:10
Conclusion
Transcript
The overall goal of this experiment is to isolate plasmacytoid dendritic cells, or PDC, with a high purity from the bone marrow of lupus-prone mice for the study of their function. This method can help answer key questions related to the character
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We report a protocol using fluorescence-activated cell sorting to isolate plasmacytoid dendritic cells (pDC) with high purity from the bone marrow of lupus-prone mice for functional studies of pDC.