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Generation of Discriminative Human Monoclonal Antibodies from Rare Antigen-specific B Cells Circulating in Blood

DOI :

10.3791/56508-v

February 6th, 2018

February 6th, 2018

10,082 Views

1CRCINA, Inserm, CNRS, Université d'Angers, Université de Nantes, 2LabEx IGO, "Immunotherapy, Graft, Oncology", 3CHU Nantes, France

We describe a method for the production of human antibodies specific for an antigen of interest, starting from rare B cells circulating in human blood. Generation of these natural antibodies is efficient and rapid, and the antibodies obtained can discriminate between highly related antigens.

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