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Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

DOI :

10.3791/61050-v

November 17th, 2020

November 17th, 2020

6,612 Views

1Translational Medicine Research Center, Ruijin Hospital North, Shanghai Jiao Tong University School of Medicine, 2Department of Microbiology and Immunology, Tongji University School of Medicine, 3Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine

This manuscript presents a protocol to induce active experimental autoimmune encephalomyelitis (EAE) in mice. A method for the isolation and characterization of the infiltrated lymphocytes in the central nervous system (CNS) is also presented to show how lymphocytes are involved in the development of CNS autoimmune disease.

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