Methods Article

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

6.9K Views

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09:01 min

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November 17th, 2020

DOI :

10.3791/61050-v

November 17th, 2020

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Zhe Ji¹^,², Chenghua Zhou¹, Hongshen Niu³, Jinshen Wang¹, Lei Shen³

¹Translational Medicine Research Center, Ruijin Hospital North, Shanghai Jiao Tong University School of Medicine, ²Department of Microbiology and Immunology, Tongji University School of Medicine, ³Shanghai Institute of Immunology, Shanghai Key Laboratory for Tumor Microenvironment and Inflammation, Shanghai Jiao Tong University School of Medicine

Summary

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Flow Cytometry

Lymphocyte Infiltration

Central Nervous System

Autoimmune Encephalomyelitis

Experimental Protocol

Tissue Homogenization

Immune Cell Analysis

Chapters in this video

0:04

Introduction

0:53

C57BL/6 Mouse Immunization

1:58

Single Brain Cell Suspension Preparation

4:19

Flow Cytometric Analysis

7:00

Results: Representative Experimental Autoimmune Encephalomyelitis (EAE) Course and Brain Lymphocyte Analyses

8:22

Conclusion

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