Results: Actin Cytoskeleton of Adherent Mammalian Cells Visualized by iPALM with Sub-20 nm Spatial Resolution in 3D
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Transcript
The overall goal of this procedure is to visualize the actin cytoskeleton in adherent mammalian cells at the ultrastructural level, using a three-dimensional super resolution microscopy method called iPALM. iPALM imaging provides sub-trending nano
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We present a protocol for the application of interferometric PhotoActivated Localization Microscopy (iPALM), a 3-dimensional single-molecule localization super resolution microscopy method, to the imaging of the actin cytoskeleton in adherent mammalian cells. This approach allows light-based visualization of nanoscale structural features that would otherwise remain unresolved by conventional diffraction-limited optical microscopy.