JoVE Logo
Faculty Resource Center

Sign In

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

DOI :

10.3791/54774-v

December 1st, 2016

December 1st, 2016

10,428 Views

1Department of Biology, South University of Science and Technology of China, Shenzhen, 2Mechanobiology Institute, Singapore, 3Department of Biomedical Engineering, National University of Singapore

We present a protocol for the application of interferometric PhotoActivated Localization Microscopy (iPALM), a 3-dimensional single-molecule localization super resolution microscopy method, to the imaging of the actin cytoskeleton in adherent mammalian cells. This approach allows light-based visualization of nanoscale structural features that would otherwise remain unresolved by conventional diffraction-limited optical microscopy.

Tags

Three dimensional Super Resolution Microscopy

-- Views

Related Videos

article

Live Cell Imaging of F-actin Dynamics via Fluorescent Speckle Microscopy (FSM)

article

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

article

Test Samples for Optimizing STORM Super-Resolution Microscopy

article

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

article

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

article

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

article

Open-source Single-particle Analysis for Super-resolution Microscopy with VirusMapper

article

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

article

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

article

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved