Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

10.6K Views

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11:57 min

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December 1st, 2016

DOI :

10.3791/54774-v

December 1st, 2016

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Yilin Wang¹, Pakorn Kanchanawong²^,³

¹Department of Biology, South University of Science and Technology of China, Shenzhen, ²Mechanobiology Institute, Singapore, ³Department of Biomedical Engineering, National University of Singapore

Summary

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Keywords 3D Super Resolution Microscopy

IPALM

F actin Filaments

Ultrastructural Visualization

Single Molecule Localization

Cell Sample Preparation

Imaging Buffer

Cover Glass Assembly

Epoxy Sealing

Newton s Rings Pattern

Chapters in this video

0:05

Title

0:55

Imaging Specimen Preparation

3:11

Sample Placement and iPALM Alignment

5:29

Calibration of the iPALM Setup

7:47

Data Acquisition

9:09

Results: Actin Cytoskeleton of Adherent Mammalian Cells Visualized by iPALM with Sub-20 nm Spatial Resolution in 3D

10:55

Conclusion

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