13.7K Views
•
10:41 min
•
January 4th, 2017
DOI :
January 4th, 2017
•Chapters in this video
0:05
Title
0:59
Amplification of the Chloramphenicol Acetyltransferase (CAT) Cassette from pBAD33 Plasmid Using Oligomers Designed for Homologous Pairing
2:38
Generation of pOX38-Tc Δgene::Cm Strains
4:26
Conjugative Mating Assay from XK1200 Donors to MC4100 Recipients
8:38
Results: Transfer Gene Knockout and Recovery of Conjugative DNA Transfer
9:45
Conclusion
Related Videos
In Vitro Transcription Assays and Their Application in Drug Discovery
15.0K Views
The Use of Induced Somatic Sector Analysis (ISSA) for Studying Genes and Promoters Involved in Wood Formation and Secondary Stem Development
8.7K Views
Analysis of Cap-binding Proteins in Human Cells Exposed to Physiological Oxygen Conditions
7.7K Views
Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
7.5K Views
Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
9.4K Views
High-Resolution Comparison of Bacterial Conjugation Frequencies
10.6K Views
Mating-based Overexpression Library Screening in Yeast
7.6K Views
Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
8.3K Views
Using Ustilago maydis as a Trojan Horse for In Situ Delivery of Maize Proteins
11.3K Views
Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
11.5K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved