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Conjugative Mating Assays for Sequence-specific Analysis of Transfer Proteins Involved in Bacterial Conjugation

13.7K Views

10:41 min

January 4th, 2017

DOI :

10.3791/54854-v

January 4th, 2017


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Conjugative Mating Assays

Chapters in this video

0:05

Title

0:59

Amplification of the Chloramphenicol Acetyltransferase (CAT) Cassette from pBAD33 Plasmid Using Oligomers Designed for Homologous Pairing

2:38

Generation of pOX38-Tc Δgene::Cm Strains

4:26

Conjugative Mating Assay from XK1200 Donors to MC4100 Recipients

8:38

Results: Transfer Gene Knockout and Recovery of Conjugative DNA Transfer

9:45

Conclusion

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