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Detection of Horizontal Gene Transfer Mediated by Natural Conjugative Plasmids in E. coli

DOI :

10.3791/64523-v

6:56 min

March 24th, 2023

March 24th, 2023

2,524 Views

1School of Biological Sciences, University of California Irvine, 2Department of Microbiology & Environmental Toxicology, University of California Santa Cruz

Conjugation mediates horizontal gene transfer by mobilizing plasmid DNA across two different cells, facilitating the spread of beneficial genes. This work describes a widely-used method for the efficient detection of conjugative plasmid transfer, based on the use of differential markers in the conjugative plasmid, donor, and recipient to detect transconjugation.

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Detection

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