In vivo Imaging of ID8 Murine Ovarian Cancer Cells
2:19
Mouse Dissection and Image Acquisition
5:04
Quantification of Tumor Burden in the Flourescence Images
9:12
Results: Quantitation of Ovarian Cancer Metastasis
10:19
Conclusion
Transcript
The overall goal of this experiment is to determine the relative tumor burden of intra-peritoneal ovarian cancer metastasis in a syngeneic orthotopic xenograft murine model. This novel method to image and quantify relative tumor burden can help us
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Ovarian cancer metastasis is characterized by numerous diffuse intra-peritoneal lesions, such that accurate visual quantitation of tumor burden is challenging. Herein we describe a method for in situ and ex vivo quantitation of metastatic tumor burden using red fluorescent protein (RFP)-labeled tumor cells and optical imaging.