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Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

DOI :

10.3791/55148-v

March 5th, 2017

March 5th, 2017

12,873 Views

1Department of Biosciences and Nutrition, Karolinska Institutet, 2School of Technology and Health, KTH Royal Institute of Technology

Many proteins perform their function when attached to membrane surfaces. The binding of extrinsic proteins on nanodisc membranes can be indirectly imaged by transmission electron microscopy. We show that the characteristic stacking (rouleau) of nanodiscs induced by the negative stain sodium phosphotungstate is prevented by the binding of extrinsic protein.

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Keywords Membrane Proteins

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