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Single Particle Cryo-Electron Microscopy: From Sample to Structure

DOI :

10.3791/62415-v

11:52 min

May 29th, 2021

May 29th, 2021

6,274 Views

1Astbury Centre Structural Molecular Biology, School Molecular and Cellular Biology, Faulty Biological Sciences, University of Leeds, 2Diamond Light Source, Harwell Science and Innovation Campus

Structure determination of macromolecular complexes using cryoEM has become routine for certain classes of proteins and complexes. Here, this pipeline is summarized (sample preparation, screening, data acquisition and processing) and readers are directed towards further detailed resources and variables that may be altered in the case of more challenging specimens.

Tags

Single particle Cryo EM

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