JoVE Logo
Faculty Resource Center

Sign In

Mitochondria and Endoplasmic Reticulum Imaging by Correlative Light and Volume Electron Microscopy

DOI :

10.3791/59750-v

July 20th, 2019

July 20th, 2019

12,275 Views

1Neural Circuits Research Group, Korea Brain Research Institute

We present a protocol to study the distribution of mitochondria and endoplasmic reticulum in whole cells after genetic modification using correlative light and volume electron microscopy including ascorbate peroxidase 2 and horseradish peroxidase staining, serial sectioning of cells with and without the target gene in the same section, and serial imaging via electron microscopy.

Tags

Mitochondria

-- Views

Related Videos

article

Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy

article

Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging

article

Analysis of β-Amyloid-induced Abnormalities on Fibrin Clot Structure by Spectroscopy and Scanning Electron Microscopy

article

Imaging of Extracellular Vesicles by Atomic Force Microscopy

article

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

article

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

article

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

article

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy

article

Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron Tomography

article

Single Particle Cryo-Electron Microscopy: From Sample to Structure

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved