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JoVE Journal

Immunology and Infection

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A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

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08:43 min

March 10th, 2017

March 10th, 2017

10,213 Views

0:05

Title

0:40

Generation of Splenocyte Cell Suspension from Nur77GFP Mouse Spleens

2:07

T Cell Isolation from the Splenocyte Cell Suspension

3:26

T-cell Activation and the Induction of GFP Expression

4:42

High Throughput Screening of Small Molecule

6:05

Results: T and B Cell Receptors Activation Flowcytometry Results and a Representative T Cell HTS of Smal Molecules

7:56

Conclusion

Transcript

The overall goal of this florescence-based lymphocyte assay is to identify new small molecule immunomodulators. This method can help answer key questions in the field of immunology about the mechanism of lymphocyte activation and inhibition. The m

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We present in the current study a novel fluorescence-based assay using lymphocytes derived from a transgenic mouse. This assay is suitable for high-throughput screening (HTS) of small molecules endowed with the capacity of either inhibiting or promoting lymphocyte activation.

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