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High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

DOI :

10.3791/50282-v

June 1st, 2014

June 1st, 2014

22,809 Views

1Department of Neurology, Massachusetts General Hospital

We present a rapid and inexpensive screening method for identifying transcriptional regulators using high-throughput robotic transfections and a homemade dual-glow luciferase assay. This protocol rapidly generates direct side-by-side functional data for thousands of genes and is easily modifiable to target any gene of interest.

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