November 6th, 2010
•We provide a reproducible method for culturing confluent monolayers of human fetal retinal pigment epithelial cells (hfRPE) cells that exhibit morphology, physiology, polarity, and protein and gene expression patterns of adult native tissue. This work has been extended to an animal model of several eye diseases.
Tags
Related Videos
Laser Capture Microdissection of Drosophila Peripheral Neurons
Dissection of a Mouse Eye for a Whole Mount of the Retinal Pigment Epithelium
Simultaneous Whole-cell Recordings from Photoreceptors and Second-order Neurons in an Amphibian Retinal Slice Preparation
Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
Olfactory Assays for Mouse Models of Neurodegenerative Disease
An In Vitro Model for the Study of Cellular Pathophysiology in Globoid Cell Leukodystrophy
Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
Low-Density Primary Hippocampal Neuron Culture
Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes
Experimental Models to Study the Neuroprotection of Acidic Postconditioning Against Cerebral Ischemia
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved