Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
21.2K Views
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13:16 min
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January 22nd, 2018
DOI :
January 22nd, 2018
•Seyyed Hamidreza Hashemipetroudi1, Ghorbanali Nematzadeh1, Gholamreza Ahmadian2, Ahad Yamchi3, Markus Kuhlmann4
1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
Chapters in this video
0:05
Title
1:35
Nucleic Acid Extraction
4:27
Validation of rDNA Based Primers with DNA Templates
6:18
gDNA Contamination Assay with RNA Templates
7:49
RT-PCR Step for cDNA Synthesis and qPCR Analysis
9:55
Results: Use of rDNA Based Primers to Detect Genomic DNA in RNA Samples of LeadTissues
12:23
Conclusion
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