Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices
13.6K Views
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12:04 min
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May 9th, 2018
DOI :
May 9th, 2018
•Vanessa Neuhaus*1, Olga Danov*1, Sebastian Konzok1, Helena Obernolte1, Susann Dehmel1, Peter Braubach2, Danny Jonigk2, Hans-Gerd Fieguth3, Patrick Zardo4, Gregor Warnecke4, Christian Martin5, Armin Braun1,6, Katherina Sewald1
1Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of REBIRTH Cluster of Excellence, 2Institute for Pathology, Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 3Division of Thoracic and Vascular Surgery, Klinikum Region Hannover (KRH), 4Department of Cardiothoracic, Transplantation and Vascular Surgery (HTTG), Hannover Medical School, German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), 5Institute of Pharmacology and Toxicology, RWTH Aachen University, 6Institute for Immunology, Hannover Medical School
Chapters in this video
0:05
Title
1:26
General Preparation of Human PCLS and Subsequent Exposure to Chemicals
4:28
Measurement of Chemically-induced Cytotoxicity in Human PCLS by WST-1 Assay
6:01
LDH Assay
7:29
Microscopic Viability Assay with Confocal Laser Scanning Microscope (cLSM)
9:49
Results: Representative Images of the Three-dimensional Microscopic Staining and Image Rendering of Human PCLS
10:59
Conclusion
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