Preparing the Tissue for RNA or Protein Lysis on PCLS
7:26
Results: Ex Vivo Pulmonary Vessel Structure and Contractility Studies of PCLS
8:32
Conclusion
Transcript
Precision cut lung slices method will preserve the structure and spatial orientations in three dimensions without regional bias and can serve as a live surrogate model for vessels and airway contractility measurements. In combination with time-lap
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Presented here is a protocol for preserving the vascular contractility of PCLS murine lung tissue, resulting in a sophisticated three-dimensional image of the pulmonary vasculature and airway, which can be preserved for up to 10 days that is susceptible to numerous procedures.