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Precision Cut Lung Slices as an Efficient Tool for Ex vivo Pulmonary Vessel Structure and Contractility Studies

DOI :

10.3791/62392-v

May 24th, 2021

May 24th, 2021

4,556 Views

1Divisions of Pulmonary Medicine, Boston Children's Hospital

Presented here is a protocol for preserving the vascular contractility of PCLS murine lung tissue, resulting in a sophisticated three-dimensional image of the pulmonary vasculature and airway, which can be preserved for up to 10 days that is susceptible to numerous procedures.

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