In Vivo Gene Delivery and Biomluminescent Imaging (BLI) and Photon Quantitification
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Pro-inflammatory Stimuli Mouse Challenge
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Results: Representative P. aeruginosa-induced Lung Inflammation Supernatent In Vivo Imaging in IL-8 Transiently Trangenic Mice
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Conclusion
Transcription
The overall goal of this procedure is to real-time monitor lung inflammation in mice after a noninvasive challenge with a pro-inflammatory stimulus such as a bacterial culture supernatant. This method can help to answer specific questions regardin
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The method described here allows for the visualization of IL-8 promoter-dependent inflammation activation in the lungs of mice through non-invasive bioluminescence imaging (BLI). The same animal can be subjected to BLI multiple times for up to two months from the time of delivery of the luciferase reporter construct.