14.1K Views
•
18:48 min
•
August 12th, 2017
DOI :
August 12th, 2017
•0:00
Title
0:37
LEW.1W to LEW.1A Allograft Procedure
2:18
Isolation of Allograft APCs (pDCs)
6:12
Isolation of Responder Effector T Cells and Regulatory T Cells (CD4+ and CD8+CD45RClow)
10:30
CFSE Labeling of Responder Cells to Measure their Proliferation
12:09
FACS Staining and Analysis After 6 Days of Coculture
13:49
In Vivo Assessment of the Cells Supressive Activity by Adoptive Cell Transfer in a Heart Grafted Recipient
16:33
Representative Results
18:03
Conclusion
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