Isolation of Responder Effector T Cells and Regulatory T Cells (CD4+ and CD8+CD45RClow)
10:30
CFSE Labeling of Responder Cells to Measure their Proliferation
12:09
FACS Staining and Analysis After 6 Days of Coculture
13:49
In Vivo Assessment of the Cells Supressive Activity by Adoptive Cell Transfer in a Heart Grafted Recipient
16:33
Representative Results
18:03
Conclusion
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Tolerance induction in transplantation is often associated with emergence of regulatory cell population. Absence of immune response to the donor, but preserved immunity against exogenous antigen. We have different protocol to assess in vitro and i
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Here, we present a protocol to induce tolerance in transplantation, and assess in vitro and in vivo the suppressive capacity of distinct cell subsets from the recipient and the immune status of the recipient toward donor or exogenous antigens.