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Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster

DOI :

10.3791/51792-v

September 20th, 2014

September 20th, 2014

25,589 Views

1Department of Biology, Indiana University

The adult structures of Drosophila are derived from sac-like structures called imaginal discs. Analysis of these discs provides insight into many developmental processes including tissue determination, compartment boundary establishment, cell proliferation, cell fate specification, and planar cell polarity. This protocol is used to prepare imaginal discs for light/fluorescent microscopy.

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Dissection

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