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Immunofluorescence Analysis of Stress Granule Formation After Bacterial Challenge of Mammalian Cells

DOI :

10.3791/55536-v

July 3rd, 2017

July 3rd, 2017

9,927 Views

1Unité de Pathogénie Microbienne Moléculaire, INSERM U786, Institut Pasteur, 2Microbiologie et Maladies Infectieuses, Collège de France, 3Laboratory of Intestinal Immunity, Institut Imagine-INSERM UMR 1163, Université Paris Descartes-Sorbonne Paris Cité

We describe a method for the qualitative and quantitative analysis of stress granule formation in mammalian cells after the cells are challenged with bacteria and a number of different stresses. This protocol can be applied to investigate the cellular stress granule response in a wide range of host-bacterial interactions.

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Immunofluorescence Analysis

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