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Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

DOI :

10.3791/50729-v

September 5th, 2013

September 5th, 2013

42,583 Views

1Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health Sciences Center

Central to the field of bacterial pathogenesis is the ability to define if and how microbes survive after exposure to eukaryotic cells. This article outlines protocols for the use of fluorescent dyes that reveal the viability of individual bacteria inside and associated with host cells.

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