JoVE Logo
Faculty Resource Center

Sign In

An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

DOI :

10.3791/55559-v

May 8th, 2017

May 8th, 2017

11,372 Views

1Public Health Research Institute Centre, New Jersey Medical School, Rutgers, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, NIAID, NIH

Here we describe a rapid equilibrium dialysis (RED) method to measure drug binding to caseum from pulmonary tuberculosis lesions and cavities. The protocol is also used with a foamy macrophage-derived matrix that is an effective surrogate to caseum.

Tags

In Vitro

-- Views

Related Videos

article

In vitro Biofilm Formation in an 8-well Chamber Slide

article

Facilitating Drug Discovery: An Automated High-content Inflammation Assay in Zebrafish

article

Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions

article

An Experimental Model to Study Tuberculosis-Malaria Coinfection upon Natural Transmission of Mycobacterium tuberculosis and Plasmodium berghei

article

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay

article

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

article

A Reference Broth Microdilution Method for Dalbavancin In Vitro Susceptibility Testing of Bacteria that Grow Aerobically

article

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

article

Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay

article

A High-throughput Compatible Assay to Evaluate Drug Efficacy against Macrophage Passaged Mycobacterium tuberculosis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved