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JoVE Journal

Immunology and Infection

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An In Vitro Caseum Binding Assay that Predicts Drug Penetration in Tuberculosis Lesions

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12:17 min

May 8th, 2017

May 8th, 2017

11,521 Views

0:05

Title

1:02

In Vitro Generation of Caseum Surrogate from THP-1 Cells

5:23

Rapid Equilibration Dialysis (RED) Assay

8:02

LC-MS Quantification and Data Analysis

9:36

Representative RED Assay Results

11:27

Conclusion

Here we describe a rapid equilibrium dialysis (RED) method to measure drug binding to caseum from pulmonary tuberculosis lesions and cavities. The protocol is also used with a foamy macrophage-derived matrix that is an effective surrogate to caseum.

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