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Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

DOI :

10.3791/55652-v

May 25th, 2017

May 25th, 2017

8,214 Views

1Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, 2Department of Anatomy, Brain Research Institute, Yonsei University College of Medicine

This protocol demonstrates a fluorescence-based method to visualize the vasculature and to quantify its complexity in Xenopus tropicalis. Blood vessels can be imaged minutes after the injection of a fluorescent dye into the beating heart of an embryo after genetic and/or pharmacological manipulations to study cardiovascular development in vivo.

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Artificial Intelligence

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