In Vitro Bioluminescence Assay to Characterize Circadian Rhythm in Mammary Epithelial Cells
9.7K Views
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11:56 min
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September 28th, 2017
DOI :
September 28th, 2017
•Chapters in this video
0:05
Title
0:53
Concstruction of PER2 Promoter Driven Destabilized Luciferase Reporter Vector
3:06
Transforming Ligated Vector pGL[hPer2P/Luc2P/Neo] to E. coli
5:28
Transient Transfection of Cells with the Circadian Vector
7:46
Establishment of the In Vitro Bioluminescence Assay in Transiently Transfected MCF10A Cells
8:52
Results: Effects of Chemicls on the Cellular Biological Clock
10:16
Conclusion
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