JoVE Logo
Faculty Resource Center

Sign In

An Optimized Hemagglutination Inhibition (HI) Assay to Quantify Influenza-specific Antibody Titers

DOI :

10.3791/55833-v

December 1st, 2017

December 1st, 2017

34,480 Views

1Applied Microbiology Research, Department of Biomedicine, University of Basel, 2Department of Biosystems Science and Engineering, ETH Zurich, 3Swiss Institute of Bioinformatics, 4Clinical Microbiology, University Hospital Basel

The presented protocols describe how to perform a hemagglutination inhibition assay to quantify influenza-specific antibody titers from serum samples of influenza vaccine recipients. The first assay determines optimal viral antigen concentrations by hemagglutination. The second assay quantifies influenza-specific antibody titers by hemagglutination inhibition.

Tags

Hemagglutination Inhibition Assay

-- Views

Related Videos

article

Methods to Quantify Pharmacologically Induced Alterations in Motor Function in Human Incomplete SCI

article

An in vivo Assay to Test Blood Vessel Permeability

article

Using Quantitative Real-time PCR to Determine Donor Cell Engraftment in a Competitive Murine Bone Marrow Transplantation Model

article

A Sensitive Method to Quantify Senescent Cancer Cells

article

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

article

Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method

article

An Optimized Evans Blue Protocol to Assess Vascular Leak in the Mouse

article

Optimized Griess Reaction for UV-Vis and Naked-eye Determination of Anti-malarial Primaquine

article

Vessel-Sparing Microsurgical Longitudinal Intussusception Vasoepididymostomy to Treat Epididymal Obstructive Azoospermia

article

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved