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Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies

DOI :

10.3791/56067-v

7:55 min

August 29th, 2017

August 29th, 2017

11,108 Views

1Department of Microbiology, Icahn School of Medicine at Mount Sinai, 2Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, 3The Department of Medicine, Section of Rheumatology, The Knapp Center for Lupus and Immunology Research, The University of Chicago

We describe a method by which we identify critical residues required for the binding of human or murine monoclonal antibodies that target the viral hemagglutinin of influenza A viruses. The protocol can be adapted to other virus surface glycoproteins and their corresponding neutralizing antibodies.

Tags

Escape Variants

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