Quantitative Analyses of all Influenza Type A Viral Hemagglutinins and Neuraminidases using Universal Antibodies in Simple Slot Blot Assays

18.2K Views

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13:12 min

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April 4th, 2011

DOI :

10.3791/2784-v

April 4th, 2011

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Caroline Gravel*¹, Changgui Li*², Junzhi Wang², Anwar M Hashem¹^,³^,⁴, Bozena Jaentschke¹, Gary Van Domselaar⁵, Runtao He⁵, Xuguang Li¹^,³

¹Centre for Vaccine Evaluation, Biologics and Genetic Therapies Directorate, HPFB, Health canada, ²National Institute for the Control of Pharmaceutical and Biological Products, The State Food and Drug Administration, Beijing, ³Department of Biochemistry, Microbiology and Immunology, University of Ottawa, ⁴Microbiology Department, Faculty of Medicine, King Abdulaziz University, ⁵National Microbiology Laboratory, Public Health Agency of Canada

Summary

Chapters in this video

0:05

Title

2:15

Identification of Hemagglutinin (HA) an Neuraminidase (NA) Conserved Regions

3:04

Slot Blot Reagent Preparation

3:53

Preparation of Influenza Vaccine Samples and Reference Standards

5:20

Assembly of the Bio-Dot SF Microfiltration Apparatus and Sample Blotting

8:08

Immunoblotting of HA and NA Antigens

9:52

Slot Blot Density Analysis

10:40

Universal Antibodies for the Quantification of HA and NA

12:10

Conclusion

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