JoVE Logo
Faculty Resource Center

Sign In

Analysis of the Gap Junction-dependent Transfer of miRNA with 3D-FRAP Microscopy

DOI :

10.3791/55870-v

June 19th, 2017

June 19th, 2017

8,671 Views

1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), 2Department of Cardiac Surgery, University of Rostock, 3Department of Life, Light and Matter of the Interdisciplinary Faculty, University of Rostock

Here, we describe the application of three-dimensional fluorescence recovery after photobleaching (3D-FRAP) for the analysis of the gap junction-dependent shuttling of miRNA. In contrast to commonly applied methods, 3D-FRAP allows for the quantification of the intercellular transfer of small RNAs in real time, with high spatio-temporal resolution.

Tags

Keywords Gap Junction

-- Views

Related Videos

article

The Xenopus Oocyte Cut-open Vaseline Gap Voltage-clamp Technique With Fluorometry

article

Detection of miRNA Targets in High-throughput Using the 3'LIFE Assay

article

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

article

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

article

Oligomerization Dynamics of Cell Surface Receptors in Living Cells by Total Internal Reflection Fluorescence Microscopy Combined with Number and Brightness Analysis

article

Tethered Bilayer Lipid Membranes to Monitor Heat Transfer between Gold Nanoparticles and Lipid Membranes

article

Application of Passive Head Motion to Generate Defined Accelerations at the Heads of Rodents

article

Measurement of Liver Stiffness Using Atomic Force Microscopy Coupled with Polarization Microscopy

article

Fluorescence Lifetime Macro Imager for Biomedical Applications

article

Advancing Mitochondrial Research – mtHyper7 Biosensor for Subcellular Analysis (Video) | JoVE

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved