Retroviral Scanning: Mapping MLV Integration Sites to Define Cell-specific Regulatory Regions
8.4K Views
•
10:10 min
•
May 28th, 2017
DOI :
May 28th, 2017
•Chapters in this video
0:05
Title
0:45
MLV Transduction of Human Cells
1:41
Restriction Enzyme Digestion and Linker Ligation
3:05
First-round Linker-mediated PCR
3:43
Second Round Linker-mediated PCR
5:17
Library Preparation and Dilution for Massive Sequencing of MLV Integration Sites
7:53
Results: Mapping MLV Integration into Epigenetically-defined Regulatory Regions
9:23
Conclusion
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