JoVE Logo
Faculty Resource Center

Sign In

CRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites

DOI :

10.3791/58572-v

November 14th, 2018

November 14th, 2018

9,529 Views

1Heinrich Pette Institute, Leibniz Institute for Experimental Virology, 2Department of Anesthesiology, University Medical Center Hamburg-Eppendorf, 3German Center for Infection Research (DZIF)

We present a genome engineering workflow for the generation of new in vitro models for HIV-1 infection that recapitulate proviral integration at selected genomic sites. Targeting of HIV-derived reporters is facilitated by CRISPR-Cas9-mediated, site-specific genome manipulation. Detailed protocols for single-cell clone generation, screening, and correct targeting verification are provided.

-- Views

Related Videos

article

Introducing Shear Stress in the Study of Bacterial Adhesion

article

Prediction of HIV-1 Coreceptor Usage (Tropism) by Sequence Analysis using a Genotypic Approach

article

Diagnosing Pulmonary Tuberculosis with the Xpert MTB/RIF Test

article

Bioluminescence Imaging of NADPH Oxidase Activity in Different Animal Models

article

Two Methods of Heterokaryon Formation to Discover HCV Restriction Factors

article

Flow Cytometric Isolation of Primary Murine Type II Alveolar Epithelial Cells for Functional and Molecular Studies

article

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

article

New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals

article

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis

article

Study of Phagolysosome Biogenesis in Live Macrophages

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved