Cell Culture, Treatment, and Examination of Protein Expression
2:53
Phosphate Assay
5:22
Microtubule-binding Assay
7:55
Localization and Expression of Tau After Treatment of Cells with Curcumin
10:23
Results: Phosphorylation, Microtubule Binding, and Localization of Tau Protein in Colorectal Cancer Cells
12:03
Conclusion
Transcript
The overall goal of this procedure is to study tau phosphorylation, the microtubule binding capabilities of tau, and localization of tau, using confocal microscopy in colorectal cancer cells. This method can be effective for most of the biological
Sign in or start your free trial to access this content
This manuscript describes standard protocols for measuring tau hyperphosphorylation, measuring tau binding to microtubules, and localization of intracellular tau following drug treatments. These protocols can be used repetitively for screening drugs or other compounds that target tau hyperphosphorylation or microtubule binding.