High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
8.7K Views
•
06:38 min
•
February 7th, 2019
DOI :
February 7th, 2019
•Chapters in this video
0:04
Title
1:00
Oligomer Annealing
1:45
Gel Preparation
3:35
Addition of the Competitor DNA Solution and Image Acquisition
4:41
Competitive Titration Assay and the Fitting Procedure
5:17
Results: Determining TF-DNA Binding Affinities Using Fluorescence Microscopy
6:07
Conclusion
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