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Quantitative Localization of a Golgi Protein by Imaging Its Center of Fluorescence Mass

DOI :

10.3791/55996-v

August 10th, 2017

August 10th, 2017

10,391 Views

1School of Biological Sciences, Nanyang Technological University, 2Bioinformatics Institute, 3School of Computing, National University of Singapore

The precise localization of Golgi residents is essential for understanding the cellular functions of the Golgi. However, conventional optical microscopy is unable to resolve the sub-Golgi structure. Here we describe the protocol for a conventional microscopy based super-resolution method to quantitatively determine the sub-Golgi localization of a protein.

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Quantitative Localization

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