Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
9.2K Views
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09:34 min
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November 27th, 2017
DOI :
November 27th, 2017
•Jaroslav Slamecka1, Javier Laurini2, Troy Shirley1, Simon Philipp Hoerstrup3, Benedikt Weber3,4,5, Laurie Owen1, Steven McClellan1
1Mitchell Cancer Institute, University of South Alabama, 2College of Medicine, University of South Alabama, 3Institute for Regenerative Medicine, University of Zurich, 4Department of Dermatology, University Hospital Zurich, 5Center for Applied Biotechnology and Molecular Medicine (CABMM), University of Zurich - Irchel Campus
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Chapters in this video
0:05
Title
0:50
Isolation of Primary Mesenchymal Stem Cells from Human Amnion
2:31
Harvesting Cultured Amniotic Fluid Stem Cells and Amniotic Membrane Stem Cells
3:47
Transfection with Reprogramming Plasmids
5:35
Picking Fully Reprogrammed Colonies for Clonial Expansion
6:43
Clonal Expansion and Maturation of iPSCs
7:47
Results: Morphological Progression of Cells During Reprogramming
8:43
Conclusion
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