Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

9.2K Views

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09:34 min

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November 27th, 2017

DOI :

10.3791/56003-v

November 27th, 2017

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Jaroslav Slamecka¹, Javier Laurini², Troy Shirley¹, Simon Philipp Hoerstrup³, Benedikt Weber³^,⁴^,⁵, Laurie Owen¹, Steven McClellan¹

¹Mitchell Cancer Institute, University of South Alabama, ²College of Medicine, University of South Alabama, ³Institute for Regenerative Medicine, University of Zurich, ⁴Department of Dermatology, University Hospital Zurich, ⁵Center for Applied Biotechnology and Molecular Medicine (CABMM), University of Zurich - Irchel Campus

Summary

Chapters in this video

0:05

Title

0:50

Isolation of Primary Mesenchymal Stem Cells from Human Amnion

2:31

Harvesting Cultured Amniotic Fluid Stem Cells and Amniotic Membrane Stem Cells

3:47

Transfection with Reprogramming Plasmids

5:35

Picking Fully Reprogrammed Colonies for Clonial Expansion

6:43

Clonal Expansion and Maturation of iPSCs

7:47

Results: Morphological Progression of Cells During Reprogramming

8:43

Conclusion

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