JoVE Logo
Faculty Resource Center

Sign In

Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

DOI :

10.3791/56004-v

September 28th, 2017

September 28th, 2017

7,880 Views

1Epigenetics Institute, University of Pennsylvania Perelman School of Medicine, 2Department of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, 3Department of Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine, 4Graduate Group in Biochemistry and Biophysics, University of Pennsylvania Perelman School of Medicine

We describe a protocol to identify RNA-binding proteins and map their RNA-binding regions in live cells using UV-mediated photocrosslinking and mass spectrometry.

Tags

Sample Preparation

-- Views

Related Videos

article

A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus

article

Capture and Identification of RNA-binding Proteins by Using Click Chemistry-assisted RNA-interactome Capture (CARIC) Strategy

article

Identification of Functional Protein Regions Through Chimeric Protein Construction

article

Nitropeptide Profiling and Identification Illustrated by Angiotensin II

article

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

article

Sample Preparation for Probe Electrospray Ionization Mass Spectrometry

article

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

article

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

article

A Sample Preparation Pipeline for Microcrystals at the VMXm Beamline

article

A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved