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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

DOI :

10.3791/64820-v

10:34 min

December 9th, 2022

December 9th, 2022

2,177 Views

1Department of Microbiology, Harvard Medical School

The protocol provides instruction for modifying RNA with dimethyl sulfate for mutational profiling experiments. It includes in vitro and in vivo probing with two alternative library preparation methods.

Tags

RNA Structure

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