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Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin

DOI :

10.3791/56027-v

July 14th, 2017

July 14th, 2017

28,826 Views

1Department of Dermatology, School of Medicine, UC San Diego

Epidermal keratinocytes form a functional skin barrier and are positioned at the front line of host defense against external environmental insults. Here we describe methods for isolation and primary culture of epidermal keratinocytes from neonatal and adult mouse skin, and induction of terminal differentiation and UVB-triggered inflammatory response from keratinocytes.

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Keywords Primary Mouse Keratinocytes

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