JoVE Logo
Faculty Resource Center

Sign In

Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy

DOI :

10.3791/56409-v

8:47 min

December 7th, 2017

December 7th, 2017

9,127 Views

1The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, 2Integrated Laboratory, Center of Translational Medicine, Taipei Medical University, 3Core Facility, Taipei Medical University, 4The Ph.D. Program for Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 5Graduate Institute of Neural Regenerative Medicine, College of Medical Science and Technology, Taipei Medical University, 6TMU research center for Neurotrauma and Neuroregeneration, College of Medical Science and Technology, Taipei Medical University

This protocol describes a technique for observation of real-time Green Fluorescence Protein (GFP) tagged Glucose Transporter 4 (GLUT4) protein trafficking upon insulin stimulation and characterization of the biological role of CCR5 in the insulin–GLUT4 signaling pathway with Deconvolution Microscopy.

Tags

GLUT4 Protein Trafficking

-- Views

Related Videos

article

Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices

article

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

article

Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices

article

Brain Slice Biotinylation: An Ex Vivo Approach to Measure Region-specific Plasma Membrane Protein Trafficking in Adult Neurons

article

Imaging Dendritic Spines of Rat Primary Hippocampal Neurons using Structured Illumination Microscopy

article

Primary Culture of Mouse Dopaminergic Neurons

article

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons

article

Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum

article

Ballistic Labeling of Pyramidal Neurons in Brain Slices and in Primary Cell Culture

article

Quantifying Spontaneous Ca2+ Fluxes and their Downstream Effects in Primary Mouse Midbrain Neurons

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved