Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

10.6K Views

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09:52 min

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December 28th, 2017

DOI :

10.3791/56436-v

December 28th, 2017

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Benjamin B. Fixman*¹, Isaac W. Babcock*¹, Laurie S. Minamide*¹, Alisa E. Shaw¹, Marina I. Oliveira da Silva¹^,², Avery M. Runyan¹, Michael T. Maloney¹^,³, Jeffrey J. Field¹, James R. Bamburg¹

¹Department of Biochemistry and Molecular Biology and Molecular, Cellular and Integrated Neuroscience Program, Colorado State University, ²IBMC-Instituto de Biologia Molecular e Celular, i3S-Instituto de Investigaçãoe Inovação em Saúde, ICBAS, Universidade do Porto, ³Denali Therapeutics

Summary

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Keywords Brain Slice Culture

Photo Etched Coverslips

Viral Vectors

Neurological Disorders

Synapse Alteration

Rodent Models

Chapters in this video

0:05

Title

1:28

Preparation of Roller Tubes and Coverslips

2:51

Plating Slices

5:44

Slice Imaging

7:28

Results: Repetitive Intermittent Imaging of Brain Slices During Long-term Culture

8:49

Conclusion

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