Results: Repetitive Intermittent Imaging of Brain Slices During Long-term Culture
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Conclusion
Transkript
The overall goal of this modified roller tube method for brain slice culture is to provide an enclosed culture system for long-term slice survival. with the capability for repetitive high-resolution florescence imaging. This method can help answer
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Presented here is a modified roller tube method for culturing and intermittent high-resolution imaging of rodent brain slices over many weeks with precise repositioning on photoetched coverslips. Neuronal viability and slice morphology are well maintained. Applications of this fully enclosed system using viruses for cell-type specific expression are provided.