JoVE Logo
Faculty Resource Center

Sign In

Isolation of Normal and Cancer-associated Fibroblasts from Fresh Tissues by Fluorescence Activated Cell Sorting (FACS)

DOI :

10.3791/4425-v

January 14th, 2013

January 14th, 2013

35,134 Views

1Department of Pathology, Sackler School of Medicine, Tel Aviv University

Cancer Associated Fibroblasts (CAFs) facilitate tumor initiation, growth and progression through signaling that promotes proliferation, angiogenesis, and inflammation. Here we describe a method to isolate pure populations of normal fibroblasts and CAFs from fresh mouse and human tissues by cell sorting, using PDGFRα as a surface marker.

Tags

Isolation

-- Views

Related Videos

article

Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting

article

Fluorescence Activated Cell Sorting of Plant Protoplasts

article

Lipid Vesicle-mediated Affinity Chromatography using Magnetic Activated Cell Sorting (LIMACS): a Novel Method to Analyze Protein-lipid Interaction

article

Bromodeoxyuridine (BrdU) Labeling and Subsequent Fluorescence Activated Cell Sorting for Culture-independent Identification of Dissolved Organic Carbon-degrading Bacterioplankton

article

Isolation of Rat Portal Fibroblasts by In situ Liver Perfusion

article

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

article

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

article

Magnetic-Activated Cell Sorting Strategies to Isolate and Purify Synovial Fluid-Derived Mesenchymal Stem Cells from a Rabbit Model

article

Single Cell Micro-aspiration as an Alternative Strategy to Fluorescence-activated Cell Sorting for Giant Virus Mixture Separation

article

Fluorescence-Activated Cell Sorting for the Isolation of Scleractinian Cell Populations

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved