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Indirect Immunofluorescence on Frozen Sections of Mouse Mammary Gland

DOI :

10.3791/53179-v

December 1st, 2015

December 1st, 2015

35,586 Views

1Jouy-en-Josas Centre, INRA

The indirect immunofluorescence protocol described in this article allows the detection and the localization of proteins in the mouse mammary gland. A complete method is given to prepare mammary gland samples, to perform immunohistochemistry, to image the tissue sections by fluorescence microscopy, and to reconstruct images.

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Keywords Indirect Immunofluorescence

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